tlr 7 8 ligand r848  (InvivoGen)

Journal: Scientific Reports

Article Title: Sex differences in innate anti-viral immune responses to respiratory viruses and in their clinical outcomes in a birth cohort study

doi: 10.1038/s41598-021-03044-x

Figure Lengend Snippet: Females have significantly greater induction than males of type I and II IFNs and CCL13/MCP4 in response to the viral mimics R848 and CpG-A. Box plots represent the 25th and 75th percentiles, the line the median, with whiskers at the 10th and 90th percentiles. Each dot represents an individual participant. Data were analysed using the Wilcoxon test. Adjusted P values: ( a ) P = 0.002, ( b ) P = 0.051, ( c ) P = 0.017 and ( d ) P = 0.104. ‘***’ 0.001 ‘**’ 0.05 ‘*’ 0.1. Data are presented as fold induction. The y axis is plotted on a logarithmic scale.

Article Snippet: We also used two viral mimics (which mimic infection with SARS-CoV-2), the Toll-like receptor (TLR)-7/8 ligand resiquimod (R848) and the TLR-9 ligand class A CpG oligonucleotide (CpG-A), both at 1 μM concentrations (Invivogen).

Techniques:

Journal: Scientific Reports

Article Title: Sex differences in innate anti-viral immune responses to respiratory viruses and in their clinical outcomes in a birth cohort study

doi: 10.1038/s41598-021-03044-x

Figure Lengend Snippet: Proportions of males and females with IFN responses to RV-A16, RSV and R848 below the 15th, 20th and 25th percentiles of the entire population. P -values are derived using chi-squared test.

Article Snippet: We also used two viral mimics (which mimic infection with SARS-CoV-2), the Toll-like receptor (TLR)-7/8 ligand resiquimod (R848) and the TLR-9 ligand class A CpG oligonucleotide (CpG-A), both at 1 μM concentrations (Invivogen).

Techniques: Derivative Assay